Verified Profile

Bio-One

Bio-One Franchise Cost & Facts · Professional Services

A Bio-One franchise costs $111K – $164K in total initial investment, with an initial franchise fee of $98K, across 127 locations. See the full Bio-One fees, royalty, and Franchise Disclosure Document below.

Building your own franchise system? See how we recruit funded franchisees.

Professional ServicesFounded 2016· 10 yrs127 unitsbiooneinc.com
Bio-One logo

Total Investment

$111K – $164K

Franchise Fee

$98K

Unit Count

127

Year Founded

2016

Overview

Why Bio-One?

Bio-One is a professional services brand with over 10 years of operating history and 127 active locations. The full Franchise Disclosure Document, including unit-level financials, territory terms, training requirements, and franchisee obligations, is available on request.

The Numbers

Investment & Fees

Total initial investment
$111K – $164K
Initial franchise fee
$98K
Royalty fee
Available on request
Ad / marketing fee
Available on request

Franchise Disclosure Documents

Bio-One FDDs

$200 per document, one-time. Pay by card and the complete PDF downloads instantly: Item 7 initial investment, Item 19 financial performance where disclosed, Item 20 outlet tables, Item 21 audited financials, and every exhibit. Latest filing on record: 2025.

Who's Operating It

81 franchisees of Bio-One

3 multi-unit operators84 units operated by franchisees
See all 81 Bio-One franchisees

Why every serious buyer reads the FDD

The Franchise Disclosure Document is the single most important piece of paperwork in your due diligence. It's federally mandated and contains everything a franchisor is required to share before you sign.

  • Item 7: full breakdown of your real startup costs
  • Item 19: actual unit-level revenue performance
  • Item 20: franchisee count, openings, closures, transfers
  • Item 21: audited financial statements

Frequently Asked

Bio-One franchise FAQ

Is Bio-One a franchise?

Yes. Bio-One is a franchise in the professional services category, which means you can own and operate a Bio-One location under a franchise agreement with the franchisor. The total initial investment to open one ranges from $111K – $164K. The franchisor provides the brand, systems, and ongoing support in exchange for an initial franchise fee and royalties, all disclosed in the Franchise Disclosure Document (FDD).

How much does it cost to open a Bio-One franchise?

Total initial investment to open a Bio-One franchise ranges from $111K – $164K according to the latest Franchise Disclosure Document. The actual cost depends on territory, build-out, and local market conditions. Unlock the full FDD on this page for Item 7's full investment breakdown.

What is the Bio-One franchise fee?

The Bio-One initial franchise fee is $98K, paid to the franchisor at signing. This is separate from total investment and does not include build-out, equipment, or working capital.

How many Bio-One franchise locations are there?

Bio-One operates 127 active locations as of the most recent FDD filing. Unlock the latest FDD for state-by-state distribution and openings/closures from Item 20.

When was Bio-One founded?

Bio-One was founded in 2016, giving the brand 10 years of operating history. Brand longevity matters in franchising because it correlates with refined operations, established supplier relationships, and a tested franchisee playbook.

Is a Bio-One franchise profitable?

Bio-One unit-level profitability depends on location, operating costs, staffing, and management. When a franchisor makes an earnings claim, it appears in Item 19 of the Franchise Disclosure Document, the only place franchise financial performance is officially disclosed. Unlock the Bio-One FDD on this page to review any Item 19 financial performance representation.

How do I apply for a Bio-One franchise opportunity?

Unlock the Bio-One Franchise Disclosure Document on this page for a flat $200. Pay by card and the complete PDF downloads instantly. The FDD contains everything you need to evaluate the opportunity: Item 7 investment, Item 19 financial performance, Item 20 unit counts and franchisee turnover, and Item 21 audited financials.

Where can I get the Bio-One FDD?

The current Bio-One FDD can be unlocked directly on this page for $200 and downloaded as a PDF immediately. Franchise Disclosure Documents are federally regulated by the FTC and must be provided to prospective franchisees at least 14 days before signing any binding agreement or paying any fee.

Compare

More Professional Services opportunities